Multiple Myeloma

 

MULTIPLE MYELOMA

Multiple myeloma (MM) is a clonal B-cell disorder characterized by malignant proliferation of monoclonal plasma cells. MM cases present common histological and morphological diagnosis, however MM displays enormous genetic and molecular complexity as well as marked variations in clinical characteristics and in patient survival. Recent progress in molecular cytogenetics has improved our understanding of pathogenesis of MM and also provided reasoning for molecular sub-classification of MM. MLPA has been shown to be a reliable technique to detect copy number alterations in MM (Alpar D. et al. 2013 and Boyle EM. et al. 2014). As balanced translocations have also a high importance in the prognostic classification of MM patients, MLPA and i-FISH are suggested to be applied as complimentary techniques in this entity.

 

This probemix contains altogether 46 probes for the following chromosomal regions and target genes 1p32-p12, 1q21-q23, 5q31, chr.9, 12p13, 13q14 (RB1-DLEU1-DIS3), 14q32 (TRAF3), chr.15, 16q12-q23 (CYLD-WWOX), 17p13 (TP53) that are suggested to be of prognostic relevance in multiple myeloma. In addition, 11 reference probes have been included in this probemix, detecting different autosomal chromosomal locations which are relatively stable by copy number in multiple myeloma.